Reporter

Part:BBa_K1740002

Designed by: tomohiko yoshida,yukina tanimura,keisuke sugahara,   Group: iGEM15_KAIT_Japan   (2015-08-25)

Red Firefly Luciferase

This Luciferase was contained in the part: BBa_K325210 that was designed by Cambridge in 2010. is a protein that catalyses the oxygenation of luciferin using ATP and molecular oxygen to yield oxyluciferin as an enzyme-adenyl-luciferin complex. In the first step, it activates luciferin as an enzyme-adenyl-luciferin complex. In the second step, luciferase acts as an oxygenase on adenyl-luciferin to produce oxyluciferin and carbon dioxide. When the second reaction proceeding, light is emitted.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1104
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1646
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1160


[edit]
Categories
Parameters
None